Direct hybridization of labeled DNA to DNA in agarose gels

Direct hybridization of labeled DNA to DNA in agarose gels

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive pro...

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Bibliographic Details
Published in:Analytical biochemistry Vol. 128; no. 2; p. 393
Main Authors: Purrello, M, Balazs, I
Format: Journal Article
Language:English
Published: United States 01-02-1983
Subjects:
Bacteriophage lambda - metabolism
Base Sequence
DNA - isolation & purification
DNA - metabolism
DNA Restriction Enzymes
Electrophoresis, Agar Gel
Molecular Weight
Nucleic Acid Hybridization
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Description
Summary:A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive probe, and the hybridization pattern visualized by autoradiography. This method is sensitive enough to detect single or low copy number DNA sequences in the genome of higher eucaryotes.
ISSN:0003-2697
DOI:10.1016/0003-2697(83)90391-3