Selective spin-labelling of an N-acetylneuraminic acid residue in the Fab-region oligosaccharide of immunoglobulin M

A method is proposed for spin-labelling using 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl, the N-acetylneuraminic acid residue within the Fab-region oligosaccharide of immunoglobulin M (IgM). The selectivity of spin-labelling was achieved by the controlled periodate oxidation of the oligosaccharide...

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Bibliographic Details
Published in:	Immunology letters Vol. 17; no. 2; p. 173
Main Authors:	Timofeev, V P, Lapuk, V A, Tchukhrova, A I, Arutiunian, A E
Format:	Journal Article
Language:	English
Published:	Netherlands 01-02-1988
Subjects:	Cyclic N-Oxides Electron Spin Resonance Spectroscopy Humans Immunoglobulin Fab Fragments Immunoglobulin M N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Spin Labels
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Summary:	A method is proposed for spin-labelling using 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl, the N-acetylneuraminic acid residue within the Fab-region oligosaccharide of immunoglobulin M (IgM). The selectivity of spin-labelling was achieved by the controlled periodate oxidation of the oligosaccharide groups of the IgM prior to the spin-labelling. The spin-labelled IgM prepared in this way may be useful for the studies of dynamics of the IgM structure, particularly upon antigen binding.
ISSN:	0165-2478
DOI:	10.1016/0165-2478(88)90087-9