Immunoglobulin isotype switching in xid mice

• Published in: Molecular immunology Vol. 32; no. 7; pp. 487 - 494
• Main Authors: Brorson, Kurt A., Krasnokutsky, Michael V., Stein, Kathryn E.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-05-1995
• Subjects: Animals; B-Lymphocytes - immunology; Female; Genetic Linkage; IgG3; immunodeficiency; immunoglobulin; Immunoglobulin G - biosynthesis; Immunoglobulin G - genetics; Immunoglobulin Isotypes - genetics; Immunoglobulin M - biosynthesis; Immunoglobulin M - genetics; Immunoglobulin Switch Region; Immunologic Deficiency Syndromes - genetics; Immunologic Deficiency Syndromes - immunology; In Vitro Techniques; isotype switch; Lymphocyte Activation; Male; Mice; Mice, Inbred CBA; Mice, Inbred DBA; Mice, Mutant Strains; RNA, Messenger - genetics; RNA, Messenger - metabolism; X Chromosome; xid; IgG3; isotype switch; xid; immunodeficiency; immunoglobulin
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/0161-5890(95)00015-7

Mice with the x-linked immunodeficiency mutation (
xid) are unresponsive to polysaccharide antigens, lack a subset of B cells, and have low serum IgM (2–20% of normal) and IgG3 (3% of normal). Because of the disproportionate reduction of IgG3, the ability of B cells from
xid mice to switch to γ3 was examined. Switching was indirectly measured by comparing IgG3 production and Cγ3 mRNA steady state levels of purified B cells activated to switch to IgG3 by LPS in bulk culture. Direct measurement of switching was achieved by enumerating on a percentage basis switched cells in a filter disk culture assay and by FACS analysis. In both bulk culture and the filter disk assay, switching to γ3 was equivalent between
xid and non-
xid B cells.