Alteration of lipoproteins of suction blister fluid by UV radiation

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 33; no. 3; pp. 233 - 238
• Main Authors: Salmon, S., Haigle, J., Bazin, M., Santus, R., Mazière, J.C., Dubertret, L.
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01-05-1996
• Subjects: Albumin; apo A-I; apo A-II; apo B; Apolipoprotein A-I - metabolism; Apolipoprotein A-I - radiation effects; Apolipoprotein A-II - metabolism; Apolipoprotein A-II - radiation effects; Apolipoprotein B-100; Apolipoproteins B - metabolism; Apolipoproteins B - radiation effects; Blister; Extracellular Space - metabolism; Extracellular Space - radiation effects; Humans; Immunotransfer; Lipid Peroxidation; Lipoproteins - metabolism; Lipoproteins - radiation effects; Photo-oxidation; SDS-PAGE; Suction; Ultraviolet Rays; SDS-PAGE; Photo-oxidation; Lipid peroxidation; Albumin; apo B; apo A-I; apo A-II; Immunotransfer
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/1011-1344(95)07260-8

Suction blister fluid is a good representative of the interstitial fluid feeding the epidermal cells. Lipoproteins contained in the suction blister fluid of healthy volunteers are readily photo-oxidized by UV radiation in the wavelength range 290–385 nm. Absorbed light doses equivalent to one minimal erythemal dose absorbbed by skin exposed to UVB (e.g. 290–320 nm) are sufficient to induce lipid peroxidation and modification of apolipoproteins A-I, A-II and B. Albumin, which is known to protect serum fully from oxidative stresses, is not so effective against photo-oxidation. Although tryptophan (Trp) residues of proteins contained in the suction blister fluid are photo-oxidized, apolipoprotein A-II, which does not contain any chromophoric Trp residue, is also altered by the UV radiation. With regard to results obtained with a model reconstituted fluid, it is suggested that the radical chain reaction of the lipid peroxidation can transfer the initial photodamage at sites not directly susceptible to photo-oxidation. The biological implications of these results are discussed.