Automated determination of neutrophil VCS parameters in diagnosis and treatment efficacy of neonatal sepsis

• Published in: Pediatric research Vol. 71; no. 1; pp. 121 - 125
• Main Authors: Celik, Istemi H., Demirel, Gamze, Aksoy, Hatice T., Erdeve, Omer, Tuncer, Ece, Biyikli, Zeynep, Dilmen, Ugur
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01-01-2012; Lippincott Williams & Wilkins
• Subjects: Bacterial diseases; Bacterial sepsis; Biological and medical sciences; clinical-investigation; Female; General aspects; Gestational Age; Hematologic Tests - instrumentation; Hematologic Tests - methods; Human bacterial diseases; Humans; Infant, Newborn; Infant, Newborn, Diseases - blood; Infant, Newborn, Diseases - diagnosis; Infant, Newborn, Diseases - therapy; Infectious diseases; Leukocyte Count; Male; Medical sciences; Medicine; Medicine & Public Health; Neutrophils - cytology; Pediatric Surgery; Pediatrics; Sensitivity and Specificity; Sepsis - blood; Sepsis - diagnosis; Sepsis - therapy; Treatment Outcome; Human; Pediatrics; Neonatal; Treatment efficiency; Granulocyte; Determination; Bacteremia; Infection; Sepsis syndrome; Newborn; Bacteriosis; Parameter; Diagnosis; Neutrophil
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/pr.2011.16

Introduction:
The Coulter LH780 hematology analyzer can evaluate mean neutrophil volume (MNV), conductivity (MNC), scatter (MNS), and distribution width (DW). We sought to investigate the value of volume, conductivity, and scatter (VCS) parameters in diagnosis and treatment efficacy of neonatal sepsis.
Results:
We observed significant increases in MNV, volume distribution width (VDW), conductivity distribution width (CDW), and significant decreases in MNC and MNS in septic newborns. There were significant decreases in MNV, VDW, and CDW, whereas MNC and MNS increased at the end of the treatment. Gram-negative sepsis caused higher MNV and VDW than Gram-positive sepsis.
Discussion:
This is the largest reported study seeking to determine cutoff levels of neutrophil VCS parameters in diagnosis of sepsis, and the first study in the evaluation of treatment efficacy and the effects of sepsis onset time and birth weight. We suggest that neutrophil VCS parameters and their DWs are useful both for early diagnosis and evaluation of treatment efficacy in neonatal sepsis without requirement for any extra blood collection.
Methods:
Peripheral blood samples from 304 newborns, 206 in group I (76 proven and 130 clinical sepsis) and 98 in group II (control group), were studied on diagnosis, 3rd day, and at the end of the treatment.