Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility?

• Published in: Andrologia Vol. 50; no. 5; pp. e12997 - n/a
• Main Authors: Mangoli, E., Khalili, M. A., Talebi, A. R., Ghasemi‐Esmailabad, S., Hosseini, A.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-06-2018
• Subjects: Acridine orange; Aniline; Biotin; Chromatin; Deoxyribonucleic acid; DNA; embryo kinetics; Embryo transfer; Infertility; intracytoplasmic sperm injection; Labeling; Oocytes; Sperm; sperm parameters; time lapse; Toluidine; Zygotes; time lapse; chromatin; embryo kinetics; intracytoplasmic sperm injection; sperm parameters
• ISSN: 0303-4569; 1439-0272
• DOI: 10.1111/and.12997

Summary
The main goal was to evaluate the correlation between sperm parameters and chromatin quality with embryo kinetics via time‐lapse monitoring system (TLM). A total of 40 couples involved in the ICSI program as a result of male infertility. For assessment of sperm chromatin and DNA quality, we used aniline blue, toluidine blue, chromomycin A3, acridine orange and terminal transferase‐mediated deoxyuridine triphosphate biotin end labelling assays. All mature oocytes were injected, and the generated zygotes (2PNs) were cultured in TLM. In day 3 after injection, single embryo transfer (SET) was carried out according to the morphology and morphokinetics. The patients were followed up until delivery. There were positive significant correlations between sperm count with CC2 (r = .330, p = .049), T4 (r = .329, p = .038), T6 (r = .342, p = .035) and T7 (r = .374, p = .025). Also, there were positive significant correlations between nonprogressive motility and T2 (r = 0.323, p = .042), T3 (r = .411, p = .013) and T4 (r = .418, p = .007). Regarding the sperm chromatin quality assays, there were negative significant correlations between CMA3 and CC2 (r = −.272, p = .049) and between acridine orange and T5 (r = −.221, p = .040). It seems that the abnormal sperm parameters and chromatin alteration affect the normal embryo kinetics in ICSI program.