Analytical quantification of low-density lipoprotein using europium tetracycline indicator

• Published in: Luminescence (Chichester, England) Vol. 24; no. 3; pp. 189 - 193
• Main Authors: De Oliveira Silva, Flávia Rodrigues, Monteiro, Andrea Moreira, Neto, Antônio M. Figueiredo, Gidlund, Magnus, Junior, Nilson Dias Vieira, Courrol, Lilia Coronato
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-05-2009
• Subjects: europium; Humans; Indicators and Reagents - analysis; Indicators and Reagents - chemistry; Lipoproteins, LDL - blood; low-density lipoprotein; Luminescence; optical spectroscopy; Organometallic Compounds - analysis; Organometallic Compounds - chemistry; Reference Values; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tetracyclines - analysis; Tetracyclines - chemistry
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.1094

Low‐density lipoprotein (LDL) is known as ‘bad’ cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator. The optical properties of the EuTc complex were investigated in aqueous solutions containing LDL. An enhancement was observed of the europium luminescence in the solutions with LDL compared those without the lipoprotein. A method to quantify the amount of LDL in a sample, based on EuTc enhanced luminescence, is proposed. The enhancement mechanism is also discussed. Copyright © 2009 John Wiley & Sons, Ltd.