Translation of HTT mRNA with expanded CAG repeats is regulated by the MID1–PP2A protein complex

• Published in: Nature communications Vol. 4; no. 1; p. 1511
• Main Authors: Krauß, Sybille, Griesche, Nadine, Jastrzebska, Ewa, Chen, Changwei, Rutschow, Désiree, Achmüller, Clemens, Dorn, Stephanie, Boesch, Sylvia M., Lalowski, Maciej, Wanker, Erich, Schneider, Rainer, Schweiger, Susann
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2013; Nature Publishing Group
• Subjects: 631/337/574; 631/378/1689/1558; 631/80/304; Animals; Blotting, Western; HeLa Cells; Humanities and Social Sciences; Humans; Huntingtin Protein; Luciferases - metabolism; Mice; Microtubule Proteins - metabolism; multidisciplinary; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Nuclear Proteins - metabolism; Nucleotide Motifs; Protein Binding; Protein Biosynthesis - genetics; Protein Phosphatase 2 - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Science; Science (multidisciplinary); TOR Serine-Threonine Kinases - metabolism; Transcription Factors - metabolism; Trinucleotide Repeat Expansion - genetics; Ubiquitin-Protein Ligases
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms2514

Expansion of CAG repeats is a common feature of various neurodegenerative disorders, including Huntington’s disease. Here we show that expanded CAG repeats bind to a translation regulatory protein complex containing MID1, protein phosphatase 2A and 40S ribosomal S6 kinase. Binding of the MID1–protein phosphatase 2A protein complex increases with CAG repeat size and stimulates translation of the CAG repeat expansion containing messenger RNA in a MID1-, protein phosphatase 2A- and mammalian target of rapamycin-dependent manner. Our data indicate that pathological CAG repeat expansions upregulate protein translation leading to an overproduction of aberrant protein and suggest that the MID1-complex may serve as a therapeutic target for the treatment of CAG repeat expansion disorders.
Expansion of CAG repeats in messenger RNAs is a common feature of various neurodegenerative disorders, including Huntington’s disease. Krauß
et al.
show that messenger RNAs with expanded CAG repeats bind to a protein complex that regulates translation and promotes overproduction of such aberrant proteins.