Insulin rapidly increases glycerol-3-phosphate-acyltransferase activity in rat adipocytes

Glycerol-3-phosphate acyltransferase (G3PAT) was activated by insulin in intact rat adipocytes within 1 min: this activation persisted for 10 min, and was due to a decrease in the Km of the enzyme. The addition of insulin to control adipocyte membranes also increased G3PAT activity, and this effect...

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Bibliographic Details
Published in:	Archives of biochemistry and biophysics Vol. 284; no. 2; p. 366
Main Authors:	Vila, M C, Farese, R V
Format:	Journal Article
Language:	English
Published:	United States 01-02-1991
Subjects:	Adipose Tissue - cytology Adipose Tissue - drug effects Adipose Tissue - enzymology Animals Cytosol - physiology Glycerol-3-Phosphate O-Acyltransferase - metabolism Insulin - pharmacology Kinetics Male Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases - pharmacology Rats Substrate Specificity
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Summary:	Glycerol-3-phosphate acyltransferase (G3PAT) was activated by insulin in intact rat adipocytes within 1 min: this activation persisted for 10 min, and was due to a decrease in the Km of the enzyme. The addition of insulin to control adipocyte membranes also increased G3PAT activity, and this effect was mimicked by phosphatidylinositol-specific phospholipase C. Cytosol fractions from insulin-treated adipocytes stimulated G3PAT activity of control membranes, suggesting that a soluble mediator is released during insulin action, possibly through activation of a PI-specific PLC.
ISSN:	0003-9861
DOI:	10.1016/0003-9861(91)90309-7