[6]-Gingerol Suppresses Oral Cancer Cell Growth by Inducing the Activation of AMPK and Suppressing the AKT/mTOR Signaling Pathway

• Published in: In vivo (Athens) Vol. 35; no. 6; pp. 3193 - 3201
• Main Authors: Zhang, Haibo, Kim, Eungyung, Yi, Junkoo, Hai, Huang, Kim, Hyeonjin, Park, Sijun, Lim, Su-Geun, Kim, Si-Yong, Jang, Soyoung, Kim, Kirim, Kim, Eun-Kyong, Lee, Youngkyun, Ryoo, Zaeyoung, Kim, Myoungok
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01-11-2021
• Subjects: AMP-Activated Protein Kinases - genetics; Apoptosis; Catechols; Cell Line, Tumor; Cell Proliferation; Fatty Alcohols; Humans; Mouth Neoplasms - drug therapy; Mouth Neoplasms - genetics; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Signal Transduction; TOR Serine-Threonine Kinases - genetics; mTOR; AMPK; AKT; Gingerol; oral cancer
• ISSN: 0258-851X; 1791-7549
• DOI: 10.21873/invivo.12614

[6]-Gingerol, a compound extracted from ginger, has been studied for its therapeutic potential in various types of cancers. However, its effects on oral cancer remain largely unknown. Here, we aimed to investigate the potential anticancer activity and underlying mechanisms of [6]-gingerol in oral cancer cells.
We analyzed the antigrowth effects of [6]-gingerol in oral cancer cell lines by cell proliferation, colony formation, migration, and invasion assays. We detected cell cycle and apoptosis with flow cytometry and further explored the mechanisms of action by immunoblotting.
[6]-Gingerol significantly inhibited oral cancer cell growth by inducing apoptosis and cell cycle G2/M phase arrest. [6]-Gingerol also inhibited oral cancer cell migration and invasion by up-regulating E-cadherin and down-regulating N-cadherin and vimentin. Moreover, [6]-gingerol induced the activation of AMPK and suppressed the AKT/mTOR signaling pathway in YD10B and Ca9-22 cells.
[6]-Gingerol exerts anticancer activity by activating AMPK and suppressing the AKT/mTOR signaling pathway in oral cancer cells. Our findings highlight the potential of [6]-gingerol as a therapeutic drug for oral cancer treatment.