Environmentally-related genotypic, phenotypic and metabolic diversity of oat (Avena sativa L.) landraces based on 67 Polish accessions

• Published in: Genetic resources and crop evolution Vol. 64; no. 8; pp. 1829 - 1840
• Main Authors: Boczkowska, Maja, Zebrowski, Jacek, Nowosielski, Jarosław, Kordulasińska, Izabela, Nowosielska, Dorota, Podyma, Wiesław
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2017; Springer Nature B.V
• Subjects: Agriculture; Avena sativa; Biomedical and Life Sciences; Coupling (molecular); DNA fingerprinting; Fingerprinting; Fourier transforms; Gene polymorphism; Genetic analysis; Genetic variability; Germplasm; Grain; Infrared spectroscopy; Life Sciences; Lipids; Metabolism; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Polymorphism; Short Communication; Spectral bands; Spectroscopy; Spectrum analysis; Structural analysis; Environment; Genetic diversity; Metabolic fingerprint; L; Phenotypic description; FTIR spectroscopy
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-017-0555-8

Here we focused on Polish oat landraces developed in the moderate temperature zone attempting to understand their genetic and phenotypic variability in context of the pressure of environmental constrains at the locations where they had evolved. Besides molecular markers and diverse phenotypic traits the Fourier transform infrared (FTIR) spectroscopy, enabling throughput metabolic fingerprinting, was used for the description of the germplasm collection. We used sophisticated multivariable analysis to integrate the data for structure analysis and to establish mutual relationships. We found that the accessions diversity across the eco-geographical variables was manifested differently depending on selected phenotypic, genetic, and metabolic criteria. Grain metabolic fingerprint derived from FTIR spectroscopy revealed the highest degree of germplasm diversity among all of the examined traits, particularly for spectral bands assigned to lipids. The landraces, which were collected in close geographical proximity, showed clear morphological and metabolic resemblances, although they represented quite different genetic backgrounds. All three levels of analysis showed the presence of selection resulting from environmental pressures and more specifically from the temperature at the landrace origin site. This research also proved that coupling of genetic polymorphism with the FTIR fingerprinting markedly extended the description of the oat landraces diversity.