An expedient synthesis of fluorescent labeled ceramide-1-phosphate analogues

A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza- s -indacene-8-yl (Me 4 -BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by...

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Bibliographic Details
Published in:	Russian journal of bioorganic chemistry Vol. 39; no. 5; pp. 539 - 542
Main Authors:	Boldyrev, I. A., Brown, R. E., Molotkovsky, J. G.
Format:	Journal Article
Language:	English
Published:	Boston Springer US 01-09-2013
Subjects:	Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Life Sciences Organic Chemistry synthesis fluorescent lipid probes phospholipase D BODIPY ceramide-1-phosphate anthrylvinyl
Online Access:	Get full text
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Summary:	A synthesis for fluorescent analogs of ceramide-1-phosphate bearing 9-anthrylvinyl or 4,4-difluoro-3a,4a-diaza- s -indacene-8-yl (Me 4 -BODIPY) fluorophore at co-position of fatty acid residue was carried out. The key stage of the synthesis is hydrolysis of corresponding sphingomyelins catalyzed by phospholipase D from Streptomyces chromofuscus ; the enzymatic yield has been raised to 50–70% by appliance of organic solvent in the incubation medium.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1068-1620 1608-330X
DOI:	10.1134/S106816201305004X