Identification and expression of Aux/IAA, ARF, and LBD family transcription factors in Boehmeria nivea

• Published in: Biologia plantarum Vol. 60; no. 2; pp. 244 - 250
• Main Authors: Huang, X., Bao, Y. N., Wang, B., Liu, L. J., Chen, J., Dai, L. J., Peng, D. X.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-06-2016; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Arabidopsis; arabidopsis thaliana; Biomedical and Life Sciences; Boehmeria nivea; high temperature; indole-acetic acid; Life Sciences; mulberry; Original Papers; phylogenetic tree; Plant Sciences; ramie; water stress; water stress; phylogenetic tree; mulberry; indole-acetic acid; high temperature; ramie
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-016-0588-4

Auxin controls numerous processes in plant development and auxin/indoleacetic acid (
Aux
/
IAA
), an auxin response factor (
ARF
), and a lateral organ boundaries domain (
LBD
) were considered as early auxin response transcription factors (TFs). Till now, no
Aux
/
IAA
,
ARF
, and
LBD
TFs were identified in ramie (
Boehmeria nivea
L. Gaud). In this study, we used
Arabidopsis
and mulberry sequences as query to search against the ramie transcriptome database and the searched sequences were analyzed for a full-length coding sequence. In total, we obtained 16
BnAux
/
IAA
, 14
BnARF
, and 16
BnLBD
TFs on which evolutionary analysis and expression profiling were conducted. Analysis of sequence conservation revealed close evolution relationships between ramie and mulberry. Expression analysis shows these genes were actively expressed in major ramie tissues, and several were auxin responsive. The expressions of these genes were also investigated under drought and a high temperature, main abiotic stresses during ramie life cycle. We found that most genes of the three families were stress-responsive and showed distinct expression patterns under the drought and high temperature stresses.