Relative roles of endothelin-1 and angiotensin II in experimental post-ischaemic acute renal failure

• Published in: Nephrology, dialysis, transplantation Vol. 19; no. 1; pp. 83 - 94
• Main Authors: Jerkić, Mirjana, Miloradović, Zoran, Jovović, Đurđdica, Mihailović-Stanojević, Nevena, Elena, Juan Vicente Rivas, Nastić-Mirić, Danica, Grujić-Adanja, Gordana, Rodríguez-Barbero, Alicia, Marković-Lipkovski, Jasmina, Vojvodić, Srećko B., Manero, Marta Vicens, Prieto, Marta Pérez, López-Novoa, José Miguel
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-01-2004
• Subjects: Acute Kidney Injury - physiopathology; Acute Kidney Injury - therapy; acute renal failure; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Angiotensin II - drug effects; Angiotensin II - physiology; Angiotensin Receptor Antagonists; Animals; Antihypertensive Agents - administration & dosage; Biological and medical sciences; Bosentan; Emergency and intensive care: renal failure. Dialysis management; Endothelin Receptor Antagonists; Endothelin-1 - drug effects; Endothelin-1 - physiology; Infusions, Intravenous; Intensive care medicine; ischaemia–reperfusion injury; Kidney - blood supply; losartan; Losartan - administration & dosage; Male; Medical sciences; Models, Animal; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Rats; Rats, Wistar; Renal failure; Reperfusion Injury - physiopathology; Reperfusion Injury - therapy; Sulfonamides - administration & dosage; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; Tetrazole derivatives; Rat; Hemodialysis; Peptide hormone; Cardiovascular disease; Non peptide compound; Extrarenal dialysis; Octapeptide; Bosentan; Reperfusion; acute renal failure; Ischemia; Losartan; Peptidergic receptor; rats; Angiotensin II; Endothelin receptor; Kidney disease; Urinary system disease; ischaemia- reperfusion injury; Rodentia; Endothelin 1; AT1 angiotensin receptor; Vertebrata; Mammalia; Biphenyl derivatives; Animal; Renal failure; Antihypertensive agent; Angiotensin antagonist
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/gfg521

Background. The relative roles of endothelin (ET)-1 and angiotensin (ANG) II in post-ischaemic acute renal failure (ARF) have not been fully established so far. With the aim of contributing to this goal, we assessed in this study the effect of ANG II and ET-1 blockade on the course of post-ischaemic-ARF. Methods. Anaesthetized Wistar rats received i.v. either bosentan (a dual ET receptor antagonist; 10 mg/kg body weight) or losartan [ANG II type 1 (AT1) receptor antagonist; 5 or 10 mg/kg body weight] or both, 20 min before, during and 20 min after ischaemia. Rats in the control group received the vehicle via the same route. Survival and renal function were monitored up to 8 days after the ischaemic challenge, while haemodynamic parameters were measured 24 h after ARF. Results. Our results demonstrate that bosentan treatment has a more beneficial effect on experimental ARF than losartan. The survival rate was remarkably higher in bosentan-treated rats than in both rat groups treated with losartan. In the ARF group treated with bosentan, renal blood flow (RBF) was increased by 129% in comparison with the untreated ARF group, whereas in the losartan-treated ARF groups, RBF was only ∼35 or 38% higher than in control ARF rats. The glomerular filtration rate was markedly higher in bosentan-treated rats than in all other ARF groups on the first and second day after ischaemia. Tubular cell injury was less severe in bosentan-treated rats than in the control ARF rats, but in losartan-treated groups it was similar to that in the ARF group. Concurrent blockade of both ET and AT1 receptors did not improve ARF because this treatment induced a marked decrease in blood pressure. Conclusions. These results suggest that ET-1 blockade is more efficient in improving the early course of post-ischaemic renal injury than ANG II inhibition, and that blockade of ET-1 might be effective in prophylaxis of ischaemic ARF.