Expression of vitellogenin receptor in the ovarian follicles during the reproductive cycle of the spotted ray Torpedo marmorata Risso 1880

• Published in: Journal of experimental zoology. Part A, Ecological genetics and physiology Vol. 315A; no. 10; pp. 585 - 592
• Main Authors: Del Giudice, Giuseppina, Prisco, Marina, Agnese, Marisa, Valiante, Salvatore, Verderame, Mariailaria, Limatola, Ermelinda, Laforgia, Vincenza, Andreuccetti, Piero
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-12-2011
• Subjects: Animals; Base Sequence; Egg Proteins - genetics; Egg Proteins - metabolism; Female; Leucoraja erinacea; Molecular Sequence Data; Oocytes - metabolism; Oogenesis - genetics; Ovarian Follicle - metabolism; Ovulation - genetics; Ovulation - metabolism; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; RNA, Messenger - metabolism; Squalus acanthias; Torpedo; Torpedo - genetics; Torpedo - metabolism; Torpedo - physiology; Torpedo marmorata
• ISSN: 1932-5223; 1932-5231; 1932-5231
• DOI: 10.1002/jez.716

The aim of this investigation was to identify the encoding sequence of vitellogenin receptor gene (vtgr), and its expression during the oogenesis in the spotted ray, Torpedo marmorata, in different phases of reproductive cycle. From an ovarian cDNA of vitellogenic female, we obtained a fragment of 581 bp, which corresponds to a partial sequence encoding the vitellogenin receptor (VTGR) in Torpedo (accession number: gi/193244760). This sequence shows a high identity with the VTGR of other vertebrates, particularly Leucoraja erinacea (89% identity) and Squalus acanthias (84% identity). We also showed that vtgr mRNA expression in the ovary modifies during the oogenesis and throughout the reproductive cycle. Indeed, in immature females, whose ovary contains only previtellogenic follicles, vtgr mRNA occurred in the oocyte cortex as well as within intermediate and pyriform cells. In mature females, whose ovary contains pre‐ and vitellogenic follicles, vtgr mRNA was detectable not only in the oocyte cortex and in intermediate and pyriform cells but also in small follicle cells present in the follicular epithelium of vitellogenic follicles. In ovulating females, that, as pregnant ones, show pre‐and vitellogenic follicles, vtgr mRNA was evident in the oocyte cortex only, whereas in pregnant females, no vtgr mRNA was evident. The role of VTGR in the control of Torpedo vitellogenesis is discussed. J. Exp. Zool. 315:585–592, 2011. © 2011 Wiley Periodicals, Inc.