Quantitative Estimation of Gymnemagenin in Gymnema sylvestre Extract and its Marketed Formulations using the HPLC-ESI-MS/MS Method

• Published in: Phytochemical analysis Vol. 24; no. 2; pp. 135 - 140
• Main Authors: Kamble, Bhagyashree, Gupta, Ankur, Patil, Dada, Janrao, Shirish, Khatal, Laxman, Duraiswamy, B.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01-02-2013; Wiley Subscription Services, Inc
• Subjects: Alkaloids - analysis; Alkaloids - chemistry; Alkaloids - standards; Chromatography, High Pressure Liquid - methods; Gymnema sylvestre; Gymnema sylvestre - chemistry; gymnemagenin; gymnemic acids; HPLC-ESI-MS/MS; Molecular Structure; Pharmaceutical Preparations - chemistry; Plant Extracts - chemistry; Quality Control; Reference Standards; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - methods; standardisation; Tandem Mass Spectrometry - methods
• ISSN: 0958-0344; 1099-1565
• DOI: 10.1002/pca.2392

ABSTRACT
Introduction
Gymnema sylvestre, with gymnemic acids as active pharmacological constituents, is a popular ayurvedic herb and has been used to treat diabetes, as a remedy for cough and as a diuretic. However, very few analytical methods are available for quality control of this herb and its marketed formulations.
Objectives
To develop and validate a new, rapid, sensitive and selective HPLC–ESI (electrospray ionisation)–MS/MS method for quantitative estimation of gymnemagenin in G. sylvestre and its marketed formulations.
Method
HPLC–ESI–MS/MS method using a multiple reactions monitoring mode was used for quantitation of gymnemagenin. Separation was carried out on a Luna C‐18 column using gradient elution of water and methanol (with 0.1% formic acid and 0.3% ammonia).
Results
The developed method was validated as per International Conference on Harmonisation Guideline ICH‐Q2B and found to be accurate, precise and linear over a relatively wide range of concentrations (5.280–305.920 ng/mL). Gymnemagenin contents were found from 0.056 ± 0.002 to 4.77 ± 0.59% w/w in G. sylvestre and its marketed formulations.
Conclusion
The method established is simple, rapid, with high sample throughput, and can be used as a tool for quality control of G. sylvestre and its formulations. Copyright © 2012 John Wiley & Sons, Ltd.
Gymnema sylvestre, with gymnemic acids as active pharmacological constituents, is a popular Ayurvedic herb and has been used as anti‐diabetic. Very few analytical methods are available for quality control of this herb. In present work, new sensitive, accurate and precise HPLC‐ESI‐MS/MS method was developed. The method was successfully applied for quantitative estimation of gymnemagenin in various marketed single as well multi‐herb formulations. Thus the method could be used as tool for quality control of G. sylvestre and its formulations.