The DNA content of human plasma cells

The DNA content of human plasma cells

The DNA content of human plasma cells from myeloma patients relative to that of leukocytes was determined by flow and microscopic cytofluorometry after propidium iodide and fluorescent Feulgen staining, respectively. Mononucleated myeloma plasma cells from all of the 17 patients studied contained mo...

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Bibliographic Details
Published in:Cancer Vol. 47; no. 10; p. 2433
Main Authors: Brox, L, Mowles, D, Pollock, B, Belch, A
Format: Journal Article
Language:English
Published: United States 15-05-1981
Subjects:
Bone Marrow - metabolism
Bone Marrow Cells
Cell Separation - methods
Chromosome Aberrations
DNA - analysis
Fluorometry
Humans
Leukocytes - analysis
Multiple Myeloma - metabolism
Plasma Cells - analysis
Staining and Labeling
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Summary:The DNA content of human plasma cells from myeloma patients relative to that of leukocytes was determined by flow and microscopic cytofluorometry after propidium iodide and fluorescent Feulgen staining, respectively. Mononucleated myeloma plasma cells from all of the 17 patients studied contained more DNA (17 to 58%) than the leukocytes from the patient. The binucleated and trinucleated plasma cells, which were more prevalent in advanced cases, contained up to two and three times, respectively, the amount of DNA determined in the mononucleated plasma cells. These observations suggest that the ploidy abnormalities of myeloma plasma cells are even more extensive than the numerous karyotypic studies have indicated.
ISSN:0008-543X
DOI:10.1002/1097-0142(19810515)47:10<2433::AID-CNCR2820471020>3.0.CO;2-D