The coasting time affects the quality of cumulus-oocyte complexes in superstimulated ewes

• Published in: Theriogenology Vol. 196; pp. 236 - 243
• Main Authors: Pinheiro, Jasmine B.S., Figueira, Lucas M., Correia, Lucas F.L., Oliveira, Thais A., Brair, Viviane L., Barros, Felipe F.P.C., Ascoli, Fabio O., Batista, Ribrio I.T.P., Brandão, Felipe Z., Souza-Fabjan, Joanna M.G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-01-2023
• Subjects: Brilliant cresyl blue; Gene expression; Oocyte quality; Ovine; Sheep; Superstimulation; Superstimulation; Ovine; Sheep; Brilliant cresyl blue; Gene expression; Oocyte quality
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2022.11.026

We hypothesized that the coasting time may be beneficial to the quality of cumulus-oocyte complexes recovered from live ewes, as reported in cattle. The present study assessed the effect of coasting times on the quantity and quality of cumulus-oocyte complexes (COCs) in sheep. All ewes were subjected to the “Day 0 protocol”, followed by an ovarian stimulation (80 mg of pFSH in three decreasing doses), varying only the coasting time [12 (G12), 36 (G36), or 60 h (G60]. In Experiment 1, data regarding follicular population was assessed. In Experiment 2, the COC quality was checked by their morphology, brilliant cresyl blue (BCB) test, evaluation of chromatin condensation pattern, and oocyte diameter. In Experiment 3, genes related to oocyte developmental competence were evaluated in BCB + COCs. The oocytes in the G60 group had more (P < 0.05) large follicles than the other groups and oocytes with a greater diameter than the G12. Oocyte morphology was similar (P > 0.05) among groups, as well as the BCB + COCs quantity. The G60-oocytes presented a better (P < 0.05) configuration of chromatin condensation compared with the other groups and a greater (P < 0.05) gene expression of BMP15, MATER, ZAR1, and PTGS2 compared with G12, and PTGS2 and HAS2 compared with G36 group. In conclusion, 60 h of coasting time positively affects the quality of COCs recovered after subjecting ewes to the “Day 0 protocol” and ovarian superstimulation. Implementing the appropriate coasting time to a given protocol can positively impact the in vitro embryo production outcomes in sheep.
•Ewes were subjected to an ovarian stimulation treatment varying only the coasting time (12, 36, or 60 h).•The G60-oocytes had more large follicles and oocytes with a larger diameter.•The G60-oocytes showed a better configuration of chromatin condensation pattern.•The G60-oocytes had a greater expression of BMP15, MATER, ZAR1, PTGS2, and HAS2.•60 h of coasting positively affects the quality of COCs from superstimulated ewes.