Protective effects of a wheat germ peptide (RVF) against H2O2-induced oxidative stress in human neuroblastoma cells

• Published in: Biotechnology letters Vol. 36; no. 8; pp. 1615 - 1622
• Main Authors: Cheng, Yunhui, Zhang, Li, Sun, Wei, Tang, Jiqing, Lv, Zhaoliang, Xu, Zhou, Yu, Huixin
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2014; Springer Nature B.V
• Subjects: Antioxidants; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cells; Hydrogen peroxide; Life Sciences; Microbiology; Original Research Paper; Oxidative stress; Wheat; Oxidative stress; Arg-Val-Phe; Human neuroblastoma cells; Wheat germ peptide (RVF); Poly(ADP-ribose) polymerase; Peptide; Apoptosis
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-014-1521-6

RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150–250 μM, 4 h) and exposed to H
2
O
2
(200 μM). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H
2
O
2
-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca
2+
dysregulation during H
2
O
2
exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.