Structural elucidation of a biologically active heteroglycan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes

• Published in: Carbohydrate research Vol. 368; pp. 22 - 28
• Main Authors: Maji, Praloy K., Sen, Ipsita K., Devi, K. Sanjana P., Maiti, Tapas K., Sikdar, Samir R., Islam, Syed S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 07-03-2013
• Subjects: Carbohydrate Sequence; carbon; Chimera; fruit extracts; fruiting bodies; galactose; glucose; Heteroglycan; Hybrid mushroom; hybrids; hydrolysis; Immunoactivation; Lentinula edodes; macrophages; Magnetic Resonance Spectroscopy; mannose; methylation; mushrooms; NMR studies; nuclear magnetic resonance spectroscopy; oxidation; Pleurotus - chemistry; Pleurotus florida; polysaccharides; Polysaccharides - chemistry; Shiitake Mushrooms - chemistry; stable isotopes; thymocytes; Heteroglycan; Hybrid mushroom; Immunoactivation; NMR studies
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2012.12.008

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► A heteroglycan was isolated from aqueous extract of a hybrid mushroom Pfle1r. ► Its structure was established by chemical and 2D NMR analysis. ► This molecule showed immunoactivation.
A water soluble heteroglycan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes consists of d-glucose, d-mannose, and d-galactose residues in a molar ratio of nearly 1:1:1. This polysaccharide showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar hydrolysis, methylation, periodate oxidation study, and NMR studies (1H, 13C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as:▪