Reference sera with graded levels of high density lipoprotein cholesterol

• Published in: Clinica chimica acta Vol. 144; no. 2-3; p. 237
• Main Authors: Kuchmak, M, Hazlehurst, J S, Olansky, A S, Taylor, L
• Format: Journal Article
• Language: English
• Published: Netherlands 29-12-1984
• Subjects: Chemical Precipitation; Cholesterol, HDL - blood; Humans; Lipoproteins, HDL - blood; Lipoproteins, LDL - blood; Reference Standards; Ultracentrifugation
• ISSN: 0009-8981
• DOI: 10.1016/0009-8981(84)90058-5

A two-step procedure for the separation of low density lipoprotein (LDL)-rich fractions and high density lipoprotein (HDL)-rich fractions from human serum is presented. The isolated precipitates are combined separately with small volumes of human serum in order to stabilize the lipoproteins. Calculated volumes of concentrates are added to human serum or to delipidated human serum [1], so that reference sera with graded levels of high density lipoprotein cholesterol (HDLC) can be prepared. These reference sera resemble human serum in appearance, composition and fractionation by ultracentrifugation. Our evaluations of the materials described in this communication have shown them to be suitable for HDLC determinations, using the precipitation procedure with heparin-manganese chloride. Properties of these reference materials are discussed.