Determination of the enantiomers of bunolol in human urine by high-performance liquid chromatography on a chiral AGP stationary phase and identification of their metabolites by gas chromatography-mass spectrometry

Determination of the enantiomers of bunolol in human urine by high-performance liquid chromatography on a chiral AGP stationary phase and identification of their metabolites by gas chromatography-mass spectrometry

A high-performance liquid chromatographic method using a chiral AGP column was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91.79-95.23% at different concentrations. The coefficients of variation (C.V.) were less tha...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Vol. 660; no. 2; p. 327
Main Authors: Li, F, Cooper, S F, Côté, M, Ayotte, C
Format: Journal Article
Language:English
Published: Netherlands 14-10-1994
Subjects:
Adult
Aging - metabolism
Chromatography, High Pressure Liquid
Gas Chromatography-Mass Spectrometry
Humans
Indicators and Reagents
Levobunolol - pharmacokinetics
Levobunolol - urine
Male
Middle Aged
Orosomucoid - chemistry
Spectrophotometry, Ultraviolet
Stereoisomerism
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Summary:A high-performance liquid chromatographic method using a chiral AGP column was developed to screen and determine the enantiomers of bunolol in human urine. The recovery of (+)- and (-)-bunolol from urine was 91.79-95.23% at different concentrations. The coefficients of variation (C.V.) were less than 2.1 and 2.3% for intra- and inter-assays, respectively. Urinary metabolites were detected using GC-MS after derivatization with N-methyl(trimethylsilyl)trifluroacetamide. The influences of pH and modifier on a chiral AGP column were studied.
ISSN:1572-6495
DOI:10.1016/0378-4347(94)00295-9