Characterization and solubilization of testosterone 17 beta-hydroxysteroid dehydrogenase in human hyperplastic prostate

• Published in: The Journal of steroid biochemistry and molecular biology Vol. 46; no. 2; p. 265
• Main Authors: Carre, J L, Quemener, E, Amet, Y, Simon, B, Berthou, F, Mangin, P, Floch, H H, Abalain, J H
• Format: Journal Article
• Language: English
• Published: England 01-08-1993
• Subjects: 17-Hydroxysteroid Dehydrogenases - chemistry; 17-Hydroxysteroid Dehydrogenases - metabolism; Detergents; Humans; Male; Prostate - enzymology; Prostatic Hyperplasia - enzymology; Solubility; Substrate Specificity; Testosterone - metabolism
• ISSN: 0960-0760
• DOI: 10.1016/0960-0760(93)90303-E

17 beta-Hydroxysteroid dehydrogenase is a membrane-bound enzyme in human prostate. Solubilization of this enzyme can only be obtained in the presence of detergents. The optimal solubilization mixture contained 50 mM Tris-HCl buffer pH 9.0, 20% glycerol, 0.1 M KCl and 5 mg/ml of the non-ionic detergent N-octyl glucoside. In these conditions, the soluble fraction contained more than 90% of the enzymatic activity. A 2.5-fold increase of specific activity was obtained during solubilization under optimal conditions.