Effects of intraluteal implants of prostaglandin E1 or E2 on angiogenic growth factors in luteal tissue of Angus and Brahman cows

• Published in: Theriogenology Vol. 82; no. 9; pp. 1224 - 1230
• Main Authors: Weems, Yoshie S., Ma, Yan, Ford, Stephen P., Nett, Terry M., Vann, Rhonda C., Lewis, Andrew W., Neuendorff, Don A., Welsh, Thomas H., Randel, Ronald D., Weems, Charles W.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2014
• Subjects: Alprostadil - pharmacology; Angiogenesis Inducing Agents - metabolism; Angiopoietin-1; Angiopoietin-1 - metabolism; Angiopoietin-2; Angiopoietin-2 - metabolism; Animals; Cattle; Corpus Luteum - drug effects; Dinoprostone - pharmacology; Drug Implants - pharmacology; Female; Fibroblast Growth Factor 2 - metabolism; Fibroblast growth factor-2; Prostaglandin E1; Prostaglandin E2; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Prostaglandin E2; Angiopoietin-1; Prostaglandin E1; Angiopoietin-2; Vascular endothelial growth factor; Fibroblast growth factor-2; Prostaglandin E
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2014.07.039

Previously, it was reported that intraluteal implants containing prostaglandin E1 or E2 (PGE1 and PGE2) in Angus or Brahman cows prevented luteolysis by preventing loss of mRNA expression for luteal LH receptors and luteal unoccupied and occupied LH receptors. In addition, intraluteal implants containing PGE1 or PGE2 upregulated mRNA expression for FP prostanoid receptors and downregulated mRNA expression for EP2 and EP4 prostanoid receptors. Luteal weight during the estrous cycle of Brahman cows was reported to be lesser than that of Angus cows but not during pregnancy. The objective of this experiment was to determine whether intraluteal implants containing PGE1 or PGE2 alter vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), angiopoietin-1 (ANG-1), and angiopoietin-2 (ANG-2) protein in Brahman or Angus cows. On Day 13 of the estrous cycle, Angus cows received no intraluteal implant and corpora lutea were retrieved, or Angus and Brahman cows received intraluteal silastic implants containing vehicle, PGE1, or PGE2 on Day 13 and corpora lutea were retrieved on Day 19. Corpora lutea slices were analyzed for VEGF, FGF-2, ANG-1, and ANG-2 angiogenic proteins via Western blot. Day-13 Angus cow luteal tissue served as preluteolytic controls. Data for VEGF were not affected (P > 0.05) by day, breed, or treatment. PGE1 or PGE2 increased (P < 0.05) FGF-2 in luteal tissue of Angus cows compared with Day-13 and Day-19 Angus controls but decreased (P < 0.05) FGF-2 in luteal tissue of Brahman cows when compared w Day-13 or Day-19 Angus controls. There was no effect (P > 0.05) of PGE1 or PGE2 on ANG-1 in Angus luteal tissue when compared with Day-13 or Day-19 controls, but ANG-1 was decreased (P < 0.05) by PGE1 or PGE2 in Brahman cows when compared with Day-19 Brahman controls. ANG-2 was increased (P < 0.05) on Day 19 in Angus Vehicle controls when compared with Day-13 Angus controls, which was prevented (P < 0.05) by PGE1 but not by PGE2 in Angus cows. There was no effect (P > 0.05) of PGE1 or PGE2 on ANG-2 in Brahman cows. PGE1 or PGE2 may alter cow luteal FGF-2, ANG-1, or ANG-2 but not VEGF to prevent luteolysis; however, species or breed differences may exist.