An automated sample preparation and high performance liquid chromatographic method for the determination of MK-591, a novel leukotriene biosynthesis inhibitor, in human plasma

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 14; no. 11; pp. 1601 - 1608
• Main Authors: Lin, Charles, Hsieh, John Y.-K., Matuszewski, Bogdan K., Dobrinska, Michael R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-08-1996; Elsevier Science
• Subjects: Analysis; Automated sample preparation; BenchMate™ Workstation; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; General pharmacology; HPLC; Humans; Indoles - analysis; Indoles - blood; Leukotriene biosynthesis inhibitor; Lipoxygenase Inhibitors - analysis; Medical sciences; MK-591; Pharmacology. Drug treatments; plasma assay; Quinolines - analysis; Quinolines - blood; Sensitivity and Specificity; Leukotriene biosynthesis inhibitor; plasma assay; Automated sample preparation; BenchMate™ Workstation; MK-591; HPLC; Human; Leukotriene; Biological fluid; Automation; Arachidonic acid derivatives; Antiinflammatory agent; HPLC chromatography; Biosynthesis; Inhibitor; Quantitative analysis; Blood plasma
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/0731-7085(96)01737-2

A high performance liquid chromatography assay utilizing an automated sample preparation procedure for the determination of a novel leukotriene biosynthesis inhibitor, (MK-591), in human plasma has been developed. After aliquoting plasma samples and adding internal standard manually, the BenchMate™ Workstation executed protein precipitation and solid-phase extraction. Following evaporation to dryness, the residue was reconstituted and chromatographed isocratically on a cyano-phase analytical column. MK-591 and the internal standard were separated from each other and from endogenous plasma substances and detected with an absorbance detector. The assay has been validated in the concentration range 10–1000 ng ml −1 and has the sensitivity and specificity necessary to quantify plasma concentrations from several clinical studies.