Dielectrophoretic study of human erythrocytes

• Published in: Journal of biomedical engineering Vol. 11; no. 5; p. 375
• Main Authors: Gopala Krishna, G, Anwar Ali, A K, Mohan, D R, Ahmad, A
• Format: Journal Article
• Language: English
• Published: England 01-09-1989
• Subjects: Electric Conductivity; Electrophoresis - methods; Electrophysiology; Erythrocytes - physiology; Humans; Models, Biological; Reference Values
• ISSN: 0141-5425
• DOI: 10.1016/0141-5425(89)90099-X

This paper is concerned with the dielectrophoretic study of human erythrocytes under cylindrical field geometry. The influence of physical variables such as the frequency and voltage of the applied electric field, conductivity of the medium in which the cells are suspended, cell concentration and exposure time of the cell to the non-uniform electric field on dielectrophoretic collection rate (DCR) is determined in a systematic manner. It is interesting to note from the DCR spectrum of human erythrocytes that the DCR is minimum at one frequency, maximum at another and there is practically no yield over a certain frequency range. This may be attributed to the variation of complex dielectric constant of the particle and medium over that frequency range. From the DCR spectrum of different groups, it is clear that DCR behaviour is different in the frequency range from 0.3-1.5 MHz, under similar conditions of temperature, conductivity and concentration of erythrocyte suspension and strength of applied AC field. The response of DCR with voltage of the applied field, concentration of cell suspension and square root of elapsed time of the cells confirms the theory of dielectrophoresis.