Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy

• Published in: Acta biochimica et biophysica Sinica Vol. 47; no. 12; pp. 969 - 980
• Main Authors: Xie, Feng, Liu, Wei, Feng, Fen, Li, Xin, He, Lu, Lv, Deguan, Qin, Xuping, Li, Lifang, Li, Lanfang, Chen, Linxi
• Format: Journal Article
• Language: English
• Published: China 01-12-2015
• Subjects: Animals; Apelin; Autophagy; Cardiomegaly - metabolism; Cell Line; Chromones - chemistry; Enzyme Inhibitors - chemistry; Extracellular Signal-Regulated MAP Kinases - metabolism; Flavonoids - chemistry; Gene Expression Regulation, Enzymologic; Intercellular Signaling Peptides and Proteins - metabolism; Morpholines - chemistry; Myocytes, Cardiac - cytology; Phosphatidylinositol 3-Kinases - metabolism; Rats; Ribosomal Protein S6 Kinases, 70-kDa - metabolism; autophagy; APJ; PI3K; apelin; cardiomyocyte hypertrophy
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmv111

Apelin is highly expressed in rat left ventricular hypertrophy Sprague Dawley rat models, and it plays a crucial role in the cardiovascular system. The aim this study was to clarify whether apelin-13 pro- motes hypertrophy in H9c2 rat cardiomyocytes and to investigate its underlying mechanism. The cardiomyocyte hypertrophy was observed by measuring the diameter, volume, and protein content of H9c2 cells. The activation of autophagy was evaluated by observing the morphology of autopha- gosomes by transmission electron microscopy, observing the subcellular localization of LC3 by light microscopy, and detecting the membrane-associated form of LC3 by western blot analysis. The phosphatidylinositol 3-kinase （PI3K） signaling pathway was identified and the proteins expression was detected using western blot analysis. The results revealed that apelin-13 increased the diameter, volume, and protein content of H9c2 cells and promoted the phosphorylation of PI3K, Akt, ERK1/2, and p70S6K. Apelin-13 activated the PI3K-Akt-ERK1/2-p70S6K pathway. PI3K inhibitor LY294002, Akt inhibitor 1701-1, ERK1/2 inhibitor PD98059 attenuated the increase of the cell diameter, volume, pro- tein content induced by apelin-13. Apelin-13 increased the autophagosomes and up-regulated the expressions of beclin 1 and LC3-11/I both transiently and stably. The autophagy inhibitor 3MA ame- liorated the increase of cell diameter, volume, and protein content that were induced by apelin-13. These results suggested that apelin-13 promotes H9c2 rat cardiomyocyte hypertrophy via PI3K- Akt-ERK1/2-p70S6K and PI3K-induced autophagy.