Physiological stabilization, community characterization, and nitrogen degradation dynamics in an anammox consortium from estuarine sediments

• Published in: Water environment research Vol. 93; no. 4; pp. 636 - 644
• Main Authors: Ronzón Bravo, Jaime J., María Cuervo‐López, Flor, Andrade Torres, Antonio, Arteaga‐Vázquez, Mario A., Martínez Hernández, Sergio
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-04-2021
• Subjects: Ammonia; Ammonia-oxidizing bacteria; Ammonium; Ammonium Compounds; Anaerobic microorganisms; Anaerobiosis; anammox consortium; Bacteria; Bacteria - genetics; Biodegradation; Bioreactors; Brackishwater environment; Candidatus Jettenia; community characterization; Consortia; Critical point; Cultures; DNA; Estuaries; estuary sediments; Humans; Incubation period; Inoculum; Microorganisms; Nitrates; Nitrites; Nitrogen; nitrogen degradation; Nitrogen removal; Nucleotide sequence; Oxidation; Oxidation-Reduction; PCR; physiological stabilization; Physiology; Sediment; Sediments; Stoichiometry; Substrates; nitrogen degradation; community characterization; anammox consortium; estuary sediments; physiological stabilization
• ISSN: 1061-4303; 1554-7531
• DOI: 10.1002/wer.1466

Anammox is a cost‐effective and sustainable process for nitrogen removal; however, the production of a physiologically stable inoculum is a critical point in the start‐up process. In this work, estuarine sediments were used as incubation seeds to obtain cultures with stable anammox activity. Assays were performed in batch cultures fed with stoichiometric amounts of ammonium and nitrite, analyzing physiological response variables and the microbial community. Estuarine sediments showed a stable anammox process after 90 days, consuming ammonium and nitrite simultaneously with concomitant generation of N2 and nitrate in stoichiometric amounts. In kinetic assays, substrates were fully consumed after 210 hr, exhibiting N2 and nitrate yields of 0.85 and 0.10, respectively. The microbial community analysis using PCR‐DGGE indicated the presence of uncultured anammox bacteria and members of the genus Candidatus Jettenia. The results evidenced the achievement of anammox cultures, although their start‐up and kinetic characteristics were less favorable than those recorded in man‐made systems.
Practitioner points
Estuarine sediments were used as incubation seeds to obtain cultures with stable anammox activity.
The sediments were fed with stoichiometric amounts of ammonium and nitrite, analyzing the physiological response variables and the microbial community.
Sediments showed a stable anammox process after 90 days, converting the substrates into N2 and nitrate according to stoichiometry.
Anammox cultures were achieved although their start‐up and kinetic characteristics were less favorable than those recorded in man‐made systems.
Microbial community analysis using PCR‐DGGE indicated the presence of uncultured anaerobic ammonia‐oxidizing bacterium and members of genus Candidatus Jettenia.
Evaluation of estuarine sediments as incubation seeds to obtain microbial cultures with stable anammox activity.