Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7

• Published in: The Journal of experimental medicine Vol. 174; no. 3; pp. 625 - 631
• Main Authors: FREEMAN, G. J, GRAY, G. S, GIMMI, C. D, LOMBARD, D. B, LIANG-JI ZHOU, WHITE, M, FINGEROTH, J. D, GRIBBEN, J. G, NADLER, L. M
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01-09-1991; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Antigenic determinants, haptens, artificial antigens; Antigens; Antigens, Differentiation, T-Lymphocyte - immunology; Antigens, Surface - genetics; Antigens, Surface - immunology; B-Lymphocytes - immunology; B-Lymphocytes - physiology; B7-1 Antigen; Biological and medical sciences; Blotting, Northern; Blotting, Southern; CD28 Antigens; Cloning, Molecular; DNA - genetics; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression; Genes; Humans; Lymphocyte Activation; Molecular immunology; Molecular Sequence Data; Oligonucleotides - chemistry; Polymerase Chain Reaction; Receptors, Immunologic - immunology; RNA, Messenger - genetics; T-Lymphocyte Subsets - immunology; Human; Cell proliferation; T cell receptor; Nucleotide sequence; Rodentia; B-Lymphocyte; Cell surface; Antigen; Signal transduction; Vertebrata; Mammalia; Gene; Mouse; T-Lymphocyte; Property structure relationship; Molecular cloning; Mechanism of action; Comparative study
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.174.3.625

Following occupancy of the T cell receptor by antigen, T cell proliferation and lymphokine production are determined by a second costimulatory signal delivered by a ligand expressed on antigen presenting cells. The human B cell activation antigen B7, which is expressed on antigen presenting cells including activated B cells and gamma interferon treated monocytes, has been shown to deliver such a costimulatory signal upon attachment to its ligand on T cells, CD28. We have cloned and sequenced the murine homologue of the human B7 gene. The predicted murine protein has 44% amino acid identity with human B7. The greatest similarity is in the Ig-V and Ig-C like domains. Murine B7 mRNA was detected in murine hematopoietic cells of B cell but not T cell origin. Cells transfected with murine B7 provided a costimulatory signal to human CD28+ T lymphocytes. These results demonstrate the costimulatory activity of murine B7 and provide evidence that the ligand attachment site is conserved between the two species.