Meningeal whole mount preparation and characterization of neural cells by flow cytometry

Meningeal whole mount preparation and characterization of neural cells by flow cytometry

Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capab...

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Bibliographic Details
Published in:Current protocols in immunology Vol. 121; no. 1; p. e50
Main Authors: Louveau, Antoine, Filiano, Anthony J, Kipnis, Jonathan
Format: Journal Article
Language:English
Published: United States 01-04-2018
Subjects:
Animals
Flow Cytometry
Meninges - cytology
Mice
Neurons - cytology
immune cells
Neuroimmunology
neurons
meninges
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Summary:Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable to diffuse into the brain parenchyma and influence the brain cells within the parenchyma, including neurons. In this unit, we will describe two protocols: analysis the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons).
ISSN:1934-368X
DOI:10.1002/cpim.50