Changes in the concentration of trigonelline in a semi-arid leguminous plant (Prosopis laevigata) induced by an arbuscular mycorrhizal fungus during the presymbiotic phase

• Published in: Mycorrhiza Vol. 13; no. 1; pp. 49 - 52
• Main Authors: ROJAS-ANDRADE, R, CERDA-GARCIA-ROJAS, C. M, FRIAS-HERNANDEZ, J. T, DENDOOVEN, L, OLALDE-PORTUGAL, V, RAMOS-VALDIVIA, A. C
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-03-2003; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Alkaloids - analysis; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Fungi - physiology; Gigaspora rosea; Mass spectrometry; Mycorrhizae - physiology; NMR; Nuclear magnetic resonance; Parasitism and symbiosis; Plant physiology and development; Plant Roots - chemistry; Plant Roots - microbiology; Prosopis - chemistry; Prosopis - microbiology; Prosopis - physiology; Prosopis laevigata; Roots; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiosis - physiology; trigonelline; Symbiosis; Trigonelline; Symbionte; Root; Semi-arid légume; Phycomycetes; Mesquite; Biosynthesis; Gigaspora rosea; Fungi; Alkaloid; Regulation(control); Prosopis laevigata; Leguminosae; Dicotyledones; Angiospermae; Mycorrhiza; Above ground plant part; Spermatophyta; Trigonellin; Thallophyta
• ISSN: 0940-6360; 1432-1890
• DOI: 10.1007/s00572-002-0201-x

An in vitro presymbiotic system between mesquite [Prosopis laevigata(Willd.) M.C. Johnst], a semi-arid leguminous plant, and pregerminated spores of Gigaspora rosea Nicol. & Schenck was established. After characteristic hyphal branching, high performance liquid chromatographic analyses of methanol extracts from P. laevigata roots revealed a concentration change in one ultraviolet-detectable product. This product was identified by nuclear magnetic resonance and mass spectrometry as trigonelline, a pyridine alkaloid. The concentration of trigonelline was constant in the aerial parts of the plant with or without G. rosea, but its concentration in the roots increased 1.8-fold when G. roseawas present. Trigonelline may be a regulatory factor during early signal events in the establishment of the arbuscular mycorrhizal symbiosis in P. laevigata.