Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells

• Published in: Biochemical and biophysical research communications Vol. 368; no. 3; pp. 543 - 549
• Main Authors: Ohsaka, Akimichi, Hirota-Komatsu, Satoko, Shibata, Miki, Ezaki, Junji, Shinohara, Fumikazu, Yoshida, Tetsuo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11-04-2008
• Subjects: Cell Differentiation; HL-60; HL-60 Cells; Humans; Macrophage differentiation; Macrophages - cytology; Macrophages - metabolism; Receptors, Vascular Endothelial Growth Factor - metabolism; Tumor angiogenesis; Up-Regulation; Vascular Endothelial Growth Factor A - metabolism; Vascular Endothelial Growth Factor C - metabolism; VEGF receptors; VEGF-C; Tumor angiogenesis; VEGF-C; HL-60; Macrophage differentiation; VEGF receptors
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.01.129

We investigated the gene expression profiles of vascular endothelial growth factor (VEGF) and its receptors in HL-60 leukemia cells. In the VEGF family, both mRNA and protein expression of VEGF-C were up-regulated in phorbol myristate acetate (PMA)-differentiated HL-60 cells. We detected two bands of ∼31 and ∼60kDa in cell lysates, and the higher expression of ∼31kDa band was further increased after stimulation with tumor necrosis factor (TNF)-α and lipopolysaccharide (LPS). A ∼31kDa VEGF-C protein was also detected in conditioned media from PMA-differentiated HL-60 cells after LPS stimulation. The mRNA expression of VEGFR-1, VEGFR-2, and neuropilin-1 (NRP-1) was markedly up-regulated in PMA-differentiated HL-60 cells, corresponding to the results from VEGF binding studies, in which VEGF binding activity was increased in PMA-differentiated HL-60 cells. These did not occur in dimethylsulfoxide (DMSO)-differentiated HL-60 cells. The expression of VEGF-C and VEGF receptors is regulated specifically in HL-60 cells during macrophage differentiation.