Characterization of anti-channel catfish IgL σ monoclonal antibodies

• Published in: Veterinary immunology and immunopathology Vol. 135; no. 3; pp. 325 - 328
• Main Authors: Edholm, Eva-Stina, Hudgens, Edward D., Tompkins, Dannielle, Sahoo, Manoranjan, Burkhalter, Bailey, Miller, Norman W., Bengtén, Eva, Wilson, Melanie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-06-2010; Amsterdam: Elsevier
• Subjects: Animals; antibodies; Antibodies, Monoclonal - immunology; Antibody Specificity; antigen-antibody reactions; Base Sequence; catfish; cell physiology; Channel catfish; cross reaction; cytokines; DNA Primers - genetics; Ictaluridae - genetics; Ictaluridae - immunology; Ictalurus punctatus; immune response; immunity; Immunoglobulin Constant Regions - chemistry; Immunoglobulin Constant Regions - genetics; Immunoglobulin Constant Regions - immunology; Immunoglobulin Isotypes - chemistry; Immunoglobulin Isotypes - genetics; Immunoglobulin Isotypes - immunology; immunoglobulin L; immunoglobulin light chain sigma; Immunoglobulin light chain σ (IgL σ); immunoglobulin light chains; Immunoglobulin Light Chains - chemistry; Immunoglobulin Light Chains - genetics; Immunoglobulin Light Chains - immunology; immunoglobulins; leukocytes; Molecular Weight; monoclonal antibodies; Monoclonal antibody; new methods; Protein Denaturation; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; validity; Channel catfish; IgH; PBL; Monoclonal antibody; Immunoglobulin light chain σ (IgL σ); IgL; mAb
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2010.01.004

This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) σ chain isotype of the channel catfish,
Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show that these anti-catfish IgL σ chain mAbs each specifically recognize the denatured form of IgL σ. Importantly, Western blotting of catfish sera using the anti-IgL σ mAbs also identified an IgL chain-sized immunoreactive band(s) of ∼27
kDa. It is anticipated that these mAbs in combination with the already existing anti-catfish Ig heavy (H) and IgL chain mAbs will be useful in future studies examining the functional roles of the different catfish IgL isotypes.