Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia

• Published in: Plant cell reports Vol. 23; no. 4; pp. 251 - 255
• Main Authors: BELLO DE CARVALHO, C. M, MAURMANN, N, LUZ, D. I, FETT-NETO, A. G, RECH, S. B
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-10-2004; Springer Nature B.V
• Subjects: Acclimatization; Acclimatization - drug effects; Acclimatization - physiology; Acetic acid; Biological and medical sciences; Biotechnology; Chromatography; Endemic plants; Endemic species; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Indoleacetic Acids - pharmacology; Indoles - pharmacology; Iridoids - metabolism; Liquid chromatography; Methods. Procedures. Technologies; Miscellaneous; Naphthaleneacetic Acids - pharmacology; Plant cells and fungal cells; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - metabolism; Plant Shoots - drug effects; Plant Shoots - growth & development; Plant Shoots - metabolism; Plant species; Valerian - drug effects; Valerian - growth & development; Valerian - metabolism; Valeriana glechomifolia; Vegetals; Auxin; Valepotriates; Dicotyledones; Angiospermae; Valeriana; Auxins; Spermatophyta; Micropropagation; Valerianaceae; Rooting; Valeriana glechomifolia
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-004-0827-6

Valeriana glechomifolia is a plant species endemic to southern Brazil that accumulates valepotriates, which are terpene derivatives, in all of its organs. Valepotriates are the presumed sedative generic components of the pharmaceutically used species of Valeriana. The influence of various concentrations of the auxins indole-3-acetic acid, indole-3-butyric acid and alpha-naphthaleneacetic acid on the growth of micropropagated V. glechomifolia was investigated under conditions of transient and continuous exposure. Changes in the development of roots and shoots as well as the production of the valepotriates acevaltrate, valtrate and didrovaltrate (analyzed by high-performance liquid chromatography) were evaluated. The best performance in valepotriate production, growth and survival under ex vitro conditions following plant acclimatization was achieved in the continuous presence of 5.71 microM IAA. When cultured in medium containing IAA plants produced stable levels of valepotriates throughout the entire cultivation period.