Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In...

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Bibliographic Details
Published in:Microorganisms (Basel) Vol. 11; no. 1; p. 202
Main Authors: Ojima-Kato, Teruyo, Nagai, Satomi, Fujita, Akane, Sakata, Junko, Tamura, Hiroto
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 12-01-2023
MDPI
Subjects:
Biomarkers
Campylobacter
Campylobacter jejuni
Culture collections
Fetuses
Food
Gene sequencing
Genetic engineering
Genomes
Guillain-Barre syndrome
Infections
Ions
MALDI-TOF MS
Microorganisms
Nucleotide sequence
Poultry
Proteins
proteotyping
Serotypes
Software
strain solution
Strains (organisms)
theoretical mass database
Japan
MALDI-TOF MS
theoretical mass database
strain solution
proteotyping
Campylobacter jejuni
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Summary:Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In this study, we constructed a theoretical mass database with the validated biomarkers to proteotype . Using 10 strains belonging to spp. available from culture collections and 41 strains isolated from humans and foods, the ribosomal protein subunits L36, L32, S14, L24, L23, L7/L12, and S11 could be selected as the effective biomarkers for the proteotyping of at MALDI-TOF MS. An accurate database of their theoretical mass-based values was constructed by matching these gene DNA sequences and the observed mass peaks. We attempted to automatically classify 41 strains isolated from nature using this database and Strain Solution ver. 2 software, and 38 strains (93%) were correctly classified into the intended group based on the theoretical mass-based values. Thus, the seven biomarkers found in this study and Strain Solution ver. 2 are promising for the proteotyping of by MALDI-TOF MS.
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ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11010202