Characterization of Lysobacter capsici strain NF87–2 and its biocontrol activities against phytopathogens

Characterization of Lysobacter capsici strain NF87–2 and its biocontrol activities against phytopathogens

Strain NF87–2 is an aerobic, non-motile and rod-shaped Gram-negative bacterium. It was isolated from the rhizosphere of green pepper. In the present study, sequence analyses of the 16S rRNA and copA genes revealed that strain NF87–2 belongs to the species Lysobacter capsici. Strain NF87–2 could prod...

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Bibliographic Details
Published in:European journal of plant pathology Vol. 155; no. 3; pp. 859 - 869
Main Authors: Liu, Youzhou, Qiao, Junqing, Liu, Yongfeng, Liang, Xuejie, Zhou, Yaqiu, Liu, Jinbing
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-11-2019
Springer Nature B.V
Subjects:
Agriculture
Antifungal activity
Bacteria
Biological control
Biomedical and Life Sciences
Cellulase
Chitinase
Damping
Disease
Ecology
Fungi
Fungicides
Fusarium oxysporum
Genes
Greenhouse effect
Greenhouses
Life Sciences
Lysobacter
Metabolites
Microorganisms
Mycelia
Original Article
Pathogens
Peptides
Phylogenetics
Plant Pathology
Plant Sciences
Rhizosphere
rRNA 16S
Secondary metabolites
Software packages
Secondary metabolites
Peptides
Copper
Biocontrol
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Summary:Strain NF87–2 is an aerobic, non-motile and rod-shaped Gram-negative bacterium. It was isolated from the rhizosphere of green pepper. In the present study, sequence analyses of the 16S rRNA and copA genes revealed that strain NF87–2 belongs to the species Lysobacter capsici. Strain NF87–2 could produce chitinase, cellulase, protease and siderophore. The strain showed a broad spectrum of antifungal activities against phytopathogens, including Alternaria brassicae , Rhizoctonia solani , Sclerotinia sclerotiorum , Botrytis cinerea , Colletotrichum gloeosporioides and Fusarium oxysporum . The secondary metabolites secreted by strain NF87–2 could inhibit the growth of both bacteria and fungi, but the mixture of peptides and proteins extracts from a suspension of strain NF87–2 could only inhibit the mycelia growth of fungi. Our results also have shown that strain NF87–2 could control pepper damping off caused by R. solani effectively in a greenhouse setting. Our findings provide a new source for a biocontrol agent and shed light on the mechanism of the antagonistic activity of L. capsici .
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-019-01817-9