Fasting augments lipid peroxidation during reperfusion after ischemia in the perfused rat liver

• Published in: Critical care medicine Vol. 27; no. 2; pp. 401 - 406
• Main Authors: Tanigawa, Koichi, Kim, Young-Myeong, Lancaster, Jack R, Zar, Harvey A
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins, Inc 01-02-1999; Lippincott
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Clinical death. Palliative care. Organ gift and preservation; Fasting - metabolism; Lipid Peroxidation; Liver - blood supply; Liver - metabolism; Luminescent Measurements; Male; Medical sciences; Prospective Studies; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion - methods; Reperfusion Injury - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Time Factors; Oxidative stress; Fasting; Rat; Liver; Rodentia; Cardiovascular disease; Hepatic disease; Lipids; Experimental study; Isolated organ; Vascular disease; Vertebrata; Mammalia; Reperfusion; Ischemia; Animal; Digestive diseases; Peroxidation
• ISSN: 0090-3493; 1530-0293
• DOI: 10.1097/00003246-199902000-00049

OBJECTIVETo test the hypothesis that fasting would aggravate postischemic lipid peroxidation in a perfused rat liver model.
DESIGNProspective, randomized study in a rat perfused liver model.
SUBJECTSMale Sprague-Dawley rats.
INTERVENTIONSLivers isolated from fed and fasted male Sprague-Dawley rats (n = 16) were exposed to 2.5 hrs of normothermic (38[degree sign]C) ischemia followed by 2 hrs of reperfusion.
MEASUREMENTS AND MAIN RESULTSLipid peroxidation was measured by chemiluminescence and thiobarbituric acid reactive substances (TBARS). Injury parameters, potassium, lactate dehydrogenase efflux, and oxygen extraction were measured every 30 mins. Chemiluminescence and TBARS were greater in the fasted ischemic group during reperfusion. (fasted vs. fedchemiluminescence, 946.8 +/- 205.5 [SEM] vs. 98.1 +/- 8.2 counts per second, p = .0004; thiobarbituric acid reactive substances, 1.11 +/- 0.25 vs. 0.21 +/- 0.032 nM/g of liver wt/min, p = .0019). Potassium efflux in the fasted group was greater than in the fed group. (1.568 +/- 0.082 vs. 1.28 +/- 0.079 [micro sign]Eq/g liver weight/min, p = .0184). Fasted livers extracted less oxygen after ischemia (1.94 +/- 0.22 vs. 1.14 +/- 0.46 microM/g liver wt/min, p = .0048). Lactate dehydrogenase levels showed no significant differences.
CONCLUSIONFasting augmented lipid peroxidation markedly. Nutrition may be an important mechanism that protects organs from oxidative injury. (Crit Care Med 1999; 27:401-406)