Efficient non-degenerate two-photon excitation for fluorescence microscopy

Efficient non-degenerate two-photon excitation for fluorescence microscopy

Non-degenerate two-photon excitation (ND-TPE) has been explored in two-photon excitation microscopy. However, a systematic study of the efficiency of ND-TPE to guide the selection of fluorophore excitation wavelengths is missing. We measured the relative non-degenerate two-photon absorption cross-se...

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Bibliographic Details
Published in:Optics express Vol. 27; no. 20; pp. 28022 - 28035
Main Authors: Sadegh, Sanaz, Yang, Mu-Han, Ferri, Christopher G L, Thunemann, Martin, Saisan, Payam A, Wei, Zhe, Rodriguez, Erik A, Adams, Stephen R, Kiliç, Kivilcim, Boas, David A, Sakadžić, Sava, Devor, Anna, Fainman, Yeshaiahu
Format: Journal Article
Language:English
Published: United States Optical Society of America 30-09-2019
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Summary:Non-degenerate two-photon excitation (ND-TPE) has been explored in two-photon excitation microscopy. However, a systematic study of the efficiency of ND-TPE to guide the selection of fluorophore excitation wavelengths is missing. We measured the relative non-degenerate two-photon absorption cross-section (ND-TPACS) of several commonly used fluorophores (two fluorescent proteins and three small-molecule dyes) and generated 2-dimensional ND-TPACS spectra. We observed that the shape of a ND-TPACS spectrum follows that of the corresponding degenerate two-photon absorption cross-section (D-TPACS) spectrum, but is higher in magnitude. We found that the observed enhancements are higher than theoretical predictions.
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ISSN:1094-4087
1094-4087
DOI:10.1364/OE.27.028022