Oxidative stress and inflammation in the pathogenesis of activated polyamine catabolism-induced acute pancreatitis

• Published in: Amino acids Vol. 33; no. 2; pp. 323 - 330
• Main Authors: Merentie, M, Uimari, A, Pietilä, M, Sinervirta, R, Keinänen, T. A, Vepsäläinen, J, Khomutov, A, Grigorenko, N, Herzig, K.-H, Jänne, J, Alhonen, L
• Format: Journal Article
• Language: English
• Published: Austria Vienna : Springer-Verlag 01-08-2007; Springer Nature B.V
• Subjects: Acetyltransferases - metabolism; Activated; Activation; Acute Disease; Amino acids; Animals; Animals, Genetically Modified; Catalase; Cytokines; Enzymes; glutathione; Inflammation - complications; Inflammatory cytokines; Interleukin-10 - blood; Interleukin-1beta - blood; Interleukin-6 - blood; lipid peroxidation; NF-kappa B - metabolism; Nitric Oxide - blood; Oxidative stress; Oxidative Stress - physiology; Pancreatitis - etiology; Pancreatitis - pathology; Polyamines; Polyamines - metabolism; Pretreatment; Rats; Rodents; Spermidine/spermine N¹-acetyltransferase; Spermine - analogs & derivatives; Spermine - pharmacology; Stresses; superoxide dismutase; Tumor Necrosis Factor-alpha - blood; Zinc
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-007-0522-3

The markers of oxidative stress and inflammation were studied in acute pancreatitis in transgenic rats exhibiting activated polyamine catabolism. In addition, the effect of bismethylspermine (Me₂Spm) pretreatment, preventing pancreatitis in this model, on these mediators was investigated. Lipid peroxidation was increased at 6 and 24 h after induction of pancreatitis. These changes as well as the markedly decreased superoxide dismutase activity at 24 h were abolished by Me₂Spm pretreatment. Glutathione level and catalase activity changed transiently, and the effect of Me₂Spm was clear at 24 h. Serum inflammatory cytokine levels increased already at 4 h whereas NF-κB was distinctly activated only at 24 h. Me₂Spm prevented the increase in TNF-α and IL-6 while it had no effect on NF-κB activation. These results show that typical inflammatory and, to a lesser degree, some oxidative stress mediators are involved and beneficially affected by the disease-ameliorating polyamine analogue in our pancreatitis model.