Short communication: Telomere lengths in different tissues of dairy cows during early and late lactation

• Published in: Journal of dairy science Vol. 99; no. 6; p. 4881
• Main Authors: Laubenthal, L, Hoelker, M, Frahm, J, Dänicke, S, Gerlach, K, Südekum, K-H, Sauerwein, H, Häussler, S
• Format: Journal Article
• Language: English
• Published: United States 01-06-2016
• Subjects: Animals; Cattle - genetics; Female; Genomics; Lactation; Liver - metabolism; Mammary Glands, Animal - metabolism; Sequence Analysis, DNA; Subcutaneous Fat - metabolism; Telomere - chemistry; Telomere - genetics; lactation; telomere length; dairy cow
• ISSN: 1525-3198
• DOI: 10.3168/jds.2015-10095

Telomeres create a protective cap on the ends of chromosomes that shorten with cell division and are influenced by stressful conditions. With the onset of lactation, high-yielding dairy cows are exposed to metabolic stress. In the present study, we aimed to analyze telomere length (TL) in key metabolic organs, such as liver, subcutaneous (sc) adipose tissue (AT), and mammary gland, as well as in peripheral blood cells during early and late lactation in German Holstein cows (n=21). Animals were fed according to their requirement, and biopsies from scAT, liver, and mammary gland as well as blood cells were collected in early and late lactation. The relative quantity of telomere products (qT), which is proportional to the average TL, was determined in genomic DNA by multiplex quantitative PCR. In this study, relative qT varied widely in the investigated tissues and blood. In late lactation, slowly proliferating tissues, such as liver and scAT, had the highest qT, whereas peripheral blood cells and in the mammary gland had the lowest qT. Comparing early with late lactation, relative qT attrition was limited to blood and mammary gland. Relationships between relative qT in blood, mammary gland, scAT, and liver suggest that blood qT might serve as a surrogate marker for tissue-specific qT. Cows with high initial qT in tissues and blood in early lactation had greater qT attrition during the course of lactation than cows with lower qT. The determination of qT could be included when phenotyping dairy cattle to test for associations with performance and fitness traits.