Direct cefepime determination in human milk using solid mercury amalgam electrode manufactured with silver nanoparticles

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 681; pp. 127 - 132
• Main Authors: Barbosa, Antonio Marcos Jacques, de Araujo, Tatiane Alfonso, Trindade, Magno Aparecido Gonçalves, Ferreira, Valdir Souza
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-08-2012
• Subjects: breast milk; Cefepime; detection limit; Electroanalysis; electrochemistry; electrodes; high performance liquid chromatography; Human milk; mercury; nanosilver; silver; Silver solid amalgam electrode; β-lactams; Human milk; β-lactams; Electroanalysis; Cefepime; Silver solid amalgam electrode
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2012.06.012

► Use of solid silver mercury amalgam electrode obtained via silver nanoparticles. ► We developed an electrochemical method to determinate cefepime in human milk sample. ► The method exhibited high sensitivity and satisfactory selectivity for CF detection.
Solid silver mercury amalgam electrodes (AgSAEs) represent a suitable alternative to mercury electrodes due to their similar electrochemical properties and non-toxicity of the amalgam material. Based on this knowledge, we report the use of silver solid amalgam as working electrode to determine cefepime in human milk sample. Square-wave voltammogram registered for cefepime in Britton–Robinson buffer at pH 2.5, presented two well-defined reduction peaks, at −0.28V (peak 1C) and −0.45V (peak 2C). Under optimized condition, the calibration curves presented linear response from 3.0×10−7 to 2.1×10−6molL−1 with limits of detection and quantification of 8.51×10−9 and 2.84×10−8molL−1, respectively. The applicability of the method was verified using spiked human milk samples and testing recovery percentages, which were ranged from 98.00% to 100.5%. The obtained results were also compared with those provided with the HPLC technique.