Quantitative diffuse reflectance spectroscopy of short-term changes in tumor oxygenation after radiation in a matched model of radiation resistance

• Published in: Biomedical optics express Vol. 9; no. 8; pp. 3794 - 3804
• Main Authors: Diaz, Paola Monterroso, Jenkins, Samir V, Alhallak, Kinan, Semeniak, Daria, Griffin, Robert J, Dings, Ruud P M, Rajaram, Narasimhan
• Format: Journal Article
• Language: English
• Published: United States Optical Society of America 01-08-2018
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.9.003794

There is a critical need to identify patients with radiation-resistant tumors early after treatment commencement. In this study, we use diffuse reflectance spectroscopy (DRS) to investigate changes in vascular oxygenation and total hemoglobin concentration in A549 radiation-sensitive and resistant tumors treated with a clinically relevant dose fraction of 2 Gy. DRS spectra were acquired before, immediately after, 24, and 48 hours after radiation. Our data reveals a significantly higher reoxygenation (sO
) in the radiation-resistant tumors 24 and 48h after treatment, and provides promising evidence that DRS can discern between the reoxygenation trends of radiation-sensitive and resistant tumors.