Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 999.09 Visual Immunoprecipitate (VIP) for Salmonella Method to the Reference Culture Method

• Published in: Journal of AOAC International Vol. 92; no. 5; pp. 1426 - 1431
• Main Authors: Feldsine, Philip T, Kerr, David E, Shen, George, Lienau, Andrew H
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01-09-2009; Oxford University Press
• Subjects: Assaying; bacterial contamination; Biological and medical sciences; Culture Media; Food adulteration and inspection; Food Analysis; Food Contamination; Food industries; Food Microbiology; food pathogens; Fundamental and applied biological sciences. Psychology; Immunoprecipitation - methods; isolation; laboratory techniques; Methods; microbial detection; plate count; precipitin tests; Reference Standards; Reproducibility of Results; Salmonella; Salmonella - isolation & purification; United States; Validation; Modification; Salmonella; Reference; Bacteria; Method; Enterobacteriaceae
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/92.5.1426

The Visual Immunoprecipitate (VIP) for the Detection of Salmonella in Foods, AOAC Official Method 999.09, has been modified to change the color of the test and control lines of the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, there were valid results from 155 samples and controls. Results showed that the modified VIP Gold for Salmonella is equivalent to the reference culture methods for the detection of Salmonella.