Anti-arboviral activity and chemical characterization of hispidulin and ethanolic extracts from Millingtonia hortensis L.f. and Oroxylum indicum (L.) Kurz (Bignoniaceae)

• Published in: Natural product research Vol. 37; no. 4; pp. 613 - 617
• Main Authors: Cardoso Reis, Adriana Cotta, Valente, Gabriel Mendonça, Silva, Breno de Mello, de Brito Magalhães, Cíntia Lopes, Kohlhoff, Markus, Brandão, Geraldo Célio
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01-02-2023
• Subjects: Bignoniaceae; Bignoniaceae - chemistry; Chemical activity; Chikungunya virus; Ethanol; Flavonoids; Flavonoids - chemistry; Indigenous species; Leaves; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Oroxylum indicum; Plant Extracts - chemistry; Plant Extracts - pharmacology; Stems; Tandem Mass Spectrometry; Vector-borne diseases; Viruses; Zika Virus; Zika Virus Infection; 6-methyl-scutellarein; Millingtonia hortensis L.f; Mayaro virus; Chikungunya virus; antiviral; Oroxylum indicum (L.) Kurz; Bignoniaceae
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2065485

L.f. and
(L.) Kurz (Bignoniaceae) are native species from the Asian continent. They are popularly used in traditional medicine and their extracts are rich in flavonoids. In this work, ethanolic extracts of stems and leaves of these species were evaluated against the Chikungunya, Zika and Mayaro virus. The extracts were subjected to analysis by ultra-efficient liquid chromatography coupled to mass spectrometry. Additionally,
leaves extract was fractionated, leading to the isolation of hispidulin. Anti-arboviral activity against the three viruses was detected for
leaves extract with EC
ranging from 37.8 to 134.1 µg/mL and for
stems extract with EC
ranging from 18.6 to 55.9 µg/mL. Hispidulin inhibited viral cytopathic effect of MAYV (EC
value 32.2 µM) and CHIKV (EC
value 78.8 µM). In LC-DAD-ESI-MS/MS analysis we characterized 25 flavonoids confirming once again the presence of these substances in extracts of these species.