Dopamine-1-receptor stimulation and mucosal tissue oxygenation in the porcine jejunum

• Published in: Critical care medicine Vol. 23; no. 9; pp. 1560 - 1566
• Main Authors: Germann, Reinhard, Hasibeder, Walter, Haisjackl, Markus, Sparr, Harald, Luz, Gabriele, Pernthaler, Heinz, Friesenecker, Barbara, Bonatti, Johannes, Gruber, Eva, Schwarz, Birgit
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Williams & Wilkins 01-09-1995; Lippincott
• Subjects: Animals; Biological and medical sciences; Digestive system; Dose-Response Relationship, Drug; Fenoldopam - pharmacology; Hemodynamics - drug effects; Intestinal Mucosa - blood supply; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Jejunum - blood supply; Jejunum - drug effects; Jejunum - metabolism; Medical sciences; Oxygen - metabolism; Oxygen Consumption - drug effects; Pharmacology. Drug treatments; Prospective Studies; Receptors, Dopamine D1 - drug effects; Receptors, Dopamine D1 - metabolism; Swine; Digestive system; Mucosa; Gut; D1 Dopamine receptor; Biological activity; Pig; Vertebrata; Mammalia; Jejunum; Animal; Dopamine agonist; Artiodactyla; Oxygenation; Ungulata
• ISSN: 0090-3493; 1530-0293
• DOI: 10.1097/00003246-199509000-00017

OBJECTIVE To evaluate the effects of dopamine-1-receptor stimulation on intestinal mucosal tissue oxygenation.
DESIGN Prospective, experimental, controlled trial.
SETTING Animal research laboratory.
SUBJECTS Anesthetized domestic pigs (30 to 45 kg).
INTERVENTIONS A small segment of the jejunal mucosa and serosa was exposed by midline laparotomy and antimesenteric incision. Fenoldopam, a selective dopamine-1-receptor agonist, was infused in steps, exponentially increasing from 0.6 to 9.6 micro gram /kg/min via a central venous catheter (n equals 8, fenoldopam group), whereas a second group (n equals 6, saline group) was only given the solvent.
MEASUREMENTS AND MAIN RESULTS Systemic hemodynamics as well as systemic and jejunal acid base and blood gas variables were measured using an arterial catheter, a thermodilution pulmonary artery catheter, and a jejunal venous catheter. Jejunal mucosal and serosal tissue PO2 were measured by means of Clark-type surface oxygen electrodes. Oxygen saturation and relative concentration of mucosal microvascular hemoglobin were measured by means of tissue reflectance spectrophotometry.In the fenoldopam group, systemic oxygen delivery (12.5 plus minus 0.8 mL/kg/min at baseline) increased by 56% (p less than .001) above baseline values. Mean arterial pressure remained unchanged. Fenoldopam produced a 51% increase in mucosal PO2 (23.8 plus minus 2.8 torr [3.2 plus minus 0.4 kPa] at baseline; p less than .001) and a 31% increase in mucosal hemoglobin oxygen saturation (55.4 plus minus 8.3% at baseline; p less than .001), but no change in serosal PO2 (58 plus minus 4.8 torr [7.7 plus minus 0.6 kPa] at baseline).
CONCLUSIONS Fenoldopam improves tissue oxygenation of the porcine jejunum in a dose-related manner. This effect is limited to the inner mucosal layer. Dopamine-1-receptor agonists should be evaluated in patients presenting with signs of intestinal mucosal ischemia.(Crit Care Med 1995; 23:1560-1566)