Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes

Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homoeologous Chromosomes

Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: pr...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1429; p. 35
Main Authors: Fominaya, Araceli, Loarce, Yolanda, González, Juan M, Ferrer, Esther
Format: Journal Article
Language:English
Published: United States 01-01-2016
Subjects:
Amides - chemistry
Avena - genetics
Chromosome Mapping - methods
Chromosomes, Plant
Fluorescent Dyes - chemistry
In Situ Hybridization, Fluorescence - methods
Tyramine - chemistry
Metaphase chromosomes
Avena
Physical mapping
TSA-FISH
Obtaining probes
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Summary:Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: probe generation and metaphase plate generation. The potential of physically mapping 12S-globulin sequences by TSA-FISH as a means of identifying homeology among chromosome regions of Avena species was tested and is discussed.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3622-9_4