Pentamethinium Salts Nanocomposite for Electrochemical Detection of Heparin

• Published in: Materials Vol. 14; no. 18; p. 5357
• Main Authors: Shishkanova, Tatiana V., Bříza, Tomáš, Řezanka, Pavel, Kejík, Zdeněk, Jakubek, Milan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 17-09-2021; MDPI
• Subjects: Aqueous solutions; Biological properties; Carbon; Electrochemical analysis; Electrodes; Electrolytes; Gold; Heparin; Hyaluronic acid; Membranes; Nanocomposites; Nanomaterials; Nanoparticles; Polymers; Polyvinyl chloride; Potassium; Selectivity; Sensors; Solvents; Spectrum analysis; Substitutes; Tetrahydrofuran; Voltammetry; Switzerland; Czech Republic; Netherlands
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma14185357

This study presents a simple route to heparin detection and develops a voltammetric approach using supramolecular principles and nanomaterials. Nanocomposites, including gold nanoparticles (AuNPs) and γ-substituted pentamethinium salts (PMS) deposited on a glass carbon (GC) electrode surface (GC/AuNPs/PMS) and covered by a plasticized poly(vinyl chloride) (PVC) membrane, are proposed for heparin detection. The conductivity of the nonconducting PVC-plasticized membrane is guaranteed by AuNPs, and the selectivity is provided by the interaction between γ-substituted PMS and anionic analytes. In order to extend the linear range, it is necessary to apply a solvent compatible with PVC-plasticized membrane, namely tetrahydrofuran. The proposed voltammetric sensor showed a concentration dependence from 1.72 up to 45.02 IU mL−1 heparin and was used for heparin detection in saline and biological samples with recovery of 95.1–100.9%.