Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde

• Published in: Technical physics Vol. 65; no. 9; pp. 1535 - 1540
• Main Authors: Nashchekina, Yu. A., Lukonina, O. A., Darvish, D. M., Nashchekin, A. V., Elokhovskii, V. Yu, Yudin, V. E., Mikhailova, N. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-09-2020; Springer; Springer Nature B.V
• Subjects: Biological properties; Classical and Continuum Physics; Collagen; Crosslinked polymers; Crosslinking; Ethylenediaminetetraacetic acid; Glutaraldehyde; Hydrogels; Mechanical properties; Physics; Physics and Astronomy; Polymer Materials for Biomedicine; Rheological properties; Rheology; Stability analysis; Toxicity
• ISSN: 1063-7842; 1090-6525
• DOI: 10.1134/S1063784220090224

We study the effect of the concentration of glutaraldehyde that can cross-link type I collagen molecules on the collagen stability, mechanical strength, and cytotoxicity. It is shown that collagen containing 10 and 18% of glutaraldehyde exhibits the highest stability in physiological conditions. The results of rheological analysis demonstrate that, for the minimal concentration of glutaraldehyde (1%), mechanical characteristics of collagen hydrogels are improved significantly. However, the glutaraldehyde concentration higher than 10% reduces the vitality of cells by 50%.