Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil

• Published in: Veterinary parasitology Vol. 87; no. 1; pp. 83 - 92
• Main Authors: Passos, D.T., Ferreira, C.A.S., da Silva, S.S., Richter, M.F., Ozaki, L.S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-11-1999
• Subjects: ADN; Animals; Blotting, Southern; BOOPHILUS MICROPLUS; BOVIN; BRASIL; BRAZIL; BRESIL; CATTLE; Cattle Diseases - parasitology; Cysteine endopeptidase; Deoxyribonuclease EcoRI - chemistry; DNA; DNA - chemistry; DNA Probes - chemistry; Female; GANADO BOVINO; Gene Library; GENETIC VARIATION; Genetic Variation - genetics; Male; Nucleic Acid Hybridization; Polymorphism; Polymorphism, Restriction Fragment Length; RFLP; Ribosomal DNA; RNA - chemistry; RNA - isolation & purification; Ticks - chemistry; Ticks - genetics; VARIACION GENETICA; VARIATION GENETIQUE; Brazil; Boophilus microplus; RFLP; Cysteine endopeptidase; Ribosomal DNA; Polymorphism
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/S0304-4017(99)00147-8

DNA from seven isolates of the cattle tick
Boophilus microplus was analyzed by restriction fragment length polymorphism (RFLP). Three different cDNA clones, named P-9, P-25 and CP-12, isolated from a
B. microplus cDNA library, were used as DNA probes. DNA sequences of P-9 have high similarity to ribosomal genes, whereas P-25 does not show significant homology with known sequences within databases. CP-12 is a cDNA clone encoding a cysteine endopeptidase gene. A limited degree of polymorphism was detected with P-9 and P-25, while CP-12 showed a different pattern of bands for each tick isolate. These findings suggest the existence of a complex genotypic diversity of the tick
B. microplus population in endemic regions.