Assessing the Antagonistic Potential and Antibiotic Resistance of Actinomycetes Isolated from Two Zheltozems of Southeastern China

• Published in: Eurasian soil science Vol. 51; no. 7; pp. 857 - 864
• Main Authors: Shirokikh, I. G., Shirokikh, A. A., Ashikhmina, T. Ya
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-07-2018; Springer; Springer Nature B.V
• Subjects: Actinomycetes; Antibiotic resistance; Antibiotics; Antifungal activity; Antifungal agents; Antiinfectives and antibacterials; Bacteria; Disease resistance; Drug resistance; Drug resistance in microorganisms; E coli; Earth and Environmental Science; Earth Sciences; Escherichia coli; Fungi; Fungicides; Genes; Geotechnical Engineering & Applied Earth Sciences; Metabolic pathways; Microbiological strains; Pathogens; Protein biosynthesis; Proteins; Pure culture; Risk assessment; Soil; Soil bacteria; Soil Biology; Soil erosion; Soil formation; Soil microbiology; Soil microorganisms; Strains (organisms); Streptomycetes; zheltozem (Cambisol); soil streptomycetes; antifungal and antibacterial activity; antibiotic resistance; antibiotics
• ISSN: 1064-2293; 1556-195X
• DOI: 10.1134/S1064229318050113

Forty streptomycete strains have been isolated into pure culture from two eroded zheltozems (yellow soils) of the Fujian province. Their antagonistic activities and contributions to the formation of soil resistome have been characterized. The estimation of the antimicrobial effect of natural isolates allowed revealing 6 strains simultaneously inhibiting the growth of the fungi
Fusarium oxisporum, Alternaria alternata, Paecilomyces
sp.,
Trichoderma
sp., and
Candida albicans
. One strain (
Streptomyces aburaviensis
F2-6) with a wide range of antifungal actions was also active against the bacteria
Pseudomonas putuda, P. fluorescens, P. sepacia, Streptococcus
sp., and
Escherichia coli
. It has been shown that the share of isolates with a wide range of antimicrobial effects (44%) and a high antagonistic activity (77%) in zheltozem of the urban Zhangzhou district is significantly higher than in the soil of the village of Hongkeng (22 and 39%, respectively). According to the resistome concept, determinants of resistance to antibiotics first appeared in their producers; they can be transmitted to other bacteria in the soil due to horizontal drift. Tests of streptomycetes for resistance to antibiotics with different acting mechanisms (inhibitors of the synthesis of cytoderm, protein, and metabolic pathways) has shown that the share of cultures with multiple resistance in zheltozems does not exceed 25–31%. No resistance to all of the studied antibiotics is characteristic of 15–16% of the strains isolated from zheltozems. The obtained data can be used for assessing the risks related to the transfer of antibiotic resistance genes from the environment to clinically valuable pathogenic species.